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The substrate Resolution was then stored at −20°C until eventually the ammonium and nitrate contents had been analyzed Using the San++ Steady-Flow Analysator (Skalar Analytic GmbH, Erkelenz, Germany). Nitrate perseverance was based on a reaction with hydrazinium sulfate, sulfanilamide and N-(one-naphthyl) ethylenediamine dihydrochloride which le